Publikationen der Promotionsstudenten im GRK1172, geordnet nach
Betreuer und Institution

Fachbereich 16: Medizin



G. Geisslinger, E. Niederberger
Novel findings in pain processing pathways: implications for miRNAs as future therapeutic targets.
Kynast KL, Russe OQ, Geisslinger G, Niederberger E. (2013). Expert Rev Neurother; 13(5):515-25.

Activation of the AMP-activated protein kinase reduces inflammatory nociception, Russe O.Q., Möser C.V.,
Kynast K.L., King, T.S. Stephan, H., Niederberger E. (2013), The Journal of Pain, 14(11):1330-40

Altered gene expression in the prefrontal cortex of young rats induced by the ADHD drug atomoxetine;
Lempp T., Toennes S., Wunder C., Russe O.Q., Möser C.V., Kynast K.L.; Freitag C., Niederberger E. (2013).
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 10;40:221-8.

NADPH oxidase-4 maintains neuropathic pain after peripheral nerve injury.
Kallenborn-Gerhardt W., Schröder K., Del Turco D., Lu R., Kynast K., Kosowski J., Niederberger E., Shah A.M.,
Brandes R.P., Geisslinger G., Schmidtko A. (2012). J Neurosci., 32(30):10136-45

The non-canonical IκB kinases IKKε and TBK1 as potential targets for the development of novel therapeutic drugs.
Niederberger E, Möser C, Kynast K, Geisslinger G. Curr Mol Med. 2012 Nov 15. [Epub ahead of print]

Modulation of central nervous system-specific microRNA-124a alters the inflammatory response in the formalin test in mice.
Kynast KL, Russe OQ, Möser CV, Geisslinger G, Niederberger E.
Pain. 2012 Nov 24. doi:pii: S0304-3959(12)00623-9. 10.1016/j.pain.2012.11.010. [Epub ahead of print]

The p21-activated kinase PAK 5 is involved in formalin-induced nociception through regulation of MAP-Kinase signaling
and formalin-specific receptors. Coste O, Möser CV, Sisignano M, Kynast KL, Minden A, Geisslinger G, Niederberger E. (2012).
Behav Brain Res. 234(1):121-8

The protein kinase IKKε is a potential target for the treatment of inflammatory hyperalgesia.
Möser CV, Kynast K, Baatz K, Russe OQ, Ferreirós N, Costiuk H, Lu R, Schmidtko A, Tegeder I, Geisslinger G,
Niederberger E. J Immunol. 2011 Sep 1;187(5):2617-25. doi: 10.4049/jimmunol.1004088. Epub 2011 Aug 3.

Nerve injury evoked loss of latexin expression in spinal cord neurons contributes to the development of neuropathic pain.
Kühlein H.N., Tegeder I., Möser C., Lim HY., Häussler A., Spieth K., Jennes I., Marschalek R., Beckhaus T., Karas M.,
Fauth M., Ehnert C., Geisslinger G., Niederberger E. (2011). PLoS One, 6(4):e19270.

MicroRNAs as new players in the pain game; Niederberger E., Kynast K., Lötsch J., Geisslinger G. (2011). Pain, 152(7):1455-8.



T. Klingebiel, U. Köhl
Tramsen L , Schmidt S, Koehl U, Huenecke S, Latgé J-P, Roeger F, Schubert R, Klingebiel T, Lehrnbecher T.
No Effect of Antifungal Compounds on Functional Properties of Human Antifungal TH1-Cells. Transpl Infect Dis (in press).

Heinze A, Elze MC, Kloess S, Ciocarlie O, Königs C, Betz S, Bremm M, Esser R, Klingebiel T, Serban M, Hutton JL, Koehl U.
Age-matched dendritic cell subpopulations reference values in childhood. Scand
J Immunol. 2013 Jan 8. doi: 10.1111/sji.12024. [Epub ahead of print]

Borchers S, Bremm M, Lehrnbecher T, Dammann E, Pabst B, Wölk B, Esser R, Yildiz M, Eder M, Stadler M, Bader P, Martin H, Jarisch A, Schneider G, Klingebiel T, Ganser A, Weissinger EM, Koehl U.
Sequential anti-cytomegalovirus response monitoring may allow prediction ofcytomegalovirus reactivation after allogeneic stem cell transplantation. PLoS One. 2012;7(12):e50248. doi: 10.1371/journal.pone.0050248. Epub 2012 Dec 13.

Brehm C, Huenecke S, Quaiser A, Esser R, Bremm M, Kloess S, Soerensen J, Kreyenberg H, Seidl C, Becker PS, Mühl H, Klingebiel T, Bader P, Passweg JR, Schwabe D, Koehl U.
IL-2 stimulated but not unstimulated NK cells induce selective disappearance of peripheral blood cells: concomitant results to a phase I/II study.PLoS One. 2011;6(11):e27351. doi: 10.1371/journal.pone.0027351. Epub 2011 Nov 9.

Bremm M, Huenecke S, Lehrnbecher T, Ponstingl E, Mueller R, Heinze A, Bug G, Quaiser A, Kapinsky M, Brehm C, Bader P, Schneider G, Klingebiel T, Koehl U.
Advanced flowcytometric analysis of regulatory T cells: CD127 downregulation early post stem cell transplantation and altered Treg/CD3(+)CD4(+)-ratio in severe GvHD or relapse. J Immunol Methods. 2011 Oct 28;373(1-2):36-44. doi: 10.1016/j.jim.2011.07.018. Epub 2011 Aug 3.

Schmidt S, Tramsen L, Hanisch M, Latgé JP, Huenecke S, Koehl U, Lehrnbecher T.
Human natural killer cells exhibit direct activity against Aspergillus fumigatus hyphae, but not against resting conidia.J Infect Dis. 2011 Feb 1;203(3):430-5. doi: 10.1093/infdis/jiq062. Epub 2010 Dec 14.

Kloess S, Huenecke S, Piechulek D, Esser R, Koch J, Brehm C, Soerensen J, Gardlowski T, Brinkmann A, Bader P, Passweg J, Klingebiel T, Schwabe D, Koehl U.
IL-2-activated haploidentical NK cells restore NKG2D-mediated NK-cell cytotoxicity in neuroblastoma patients by scavenging of plasma MICA. EurJ Immunol. 2010 Nov;40(11):3255-67. doi: 10.1002/eji.201040568. Epub 2010 Oct 27.

Koenig M, Huenecke S, Salzmann E, Esser R, Quaritsch R, Steinhilber D, Radeke HH, Martin H, Bader P, Klingebiel T, Schwabe D, Schneider G, Lehrnbecher T, Orth A, Koehl U.
Multivariate analyses of immune reconstitution in children following allogeneic stem cell transplantation: Risk-estimation based on age-matched leukocyte subpopulations.Bone Marrow Transplant, 45(4):613-621, Apr 2010

Huenecke S, Zimmermann SY, Kloess S, Esser R, Brinkmann A, Tramsen L, Koenig M, Erben S, Seidl C, Tonn T, Eggert A, Schramm A, Bader P, Klingebiel T, Lehrnbecher T, Passweg JR, Soerensen J, Schwabe D, Koehl U.
IL-2-driven regulation of NK cell receptors with regard to the distribution of CD16+ and CD16- subpopulations and in vivo influence after haploidentical NK cell infusion. J Immunother. 2010 Feb-Mar;33(2):200-10.

Kuçi S, Kuçi Z, Kreyenberg H, Deak E, Pütsch K, Huenecke S, Amara C, Koller S, Rettinger E, Grez M, Koehl U, Latifi-Pupovci H, Henschler R, Tonn T, von Laer D, Klingebiel T, Bader P.
CD271 antigen defines a subset of multipotent stromal cells with immunosuppressive and lymphohematopoietic engraftment-promoting properties. Haematologica. 2010 Apr;95(4):651-9. Epub 2010 Feb 23.

Lehrnbecher T, Schubert R, Behl M, Koenig M, Rose M A, Koehl U, Meisel R, Laws H J.
Impaired pneumococcal immunity in children after treatment for acute lymphoblastic leukaemia. Br J Haematol, 147(5):700-705, Dec 2009.

Huenecke S, Behl M, Fadler C, Zimmermann SY, Bochennek K, Tramsen L, Esser R, Klarmann D, Kamper M, Sattler A, von Laer D, Klingebiel T, Lehrnbecher T, Koehl U.
Age-matched lymphocyte subpopulation reference values in childhood and adolescence: application of exponential regression analysis. Eur J Haematol 80:532-9, 2008.

Hollatz G, Grez M, Mastaglio S, Quaritsch R, Huenecke S, Ciceri F, Bonini C, Esser R, Klingebiel T, Kreuter J, Koehl U.
T cells for suicide gene therapy: Activation, functionality and clinical relevance. J Immunol Methods 29;331(1-2):69-81, 2008.

Koehl U, Dirkwinkel E, Koenig M, Erben S, Soerensen J, Bader P, Doerr HW, Preiser W, Weissinger E, Klingebiel T, Martin H, Lehrnbecher T.
Reconstitution of Cytomegalovirus Specific T Cells after Pediatric Allogeneic Stem Cell Transplantation: Results from a Pilot Study Using a Multi-Allele CMV Tetramer Group. Klinische Pädiatrie 220(6):348-52, 2008.

Klöss S, Bochennek K, Huenecke S, Zimmermann SY, Kuçi S, Müller T, Wels WS, Klingebiel T, Esser R, Koehl U.
A novel five-colour flow cytometric assay to determine NK cell cytotoxicity against neuroblastoma and other adherent tumour cells. J Immunol Methods 325:140-147, 2007.



J. Pfeilschifter/S. Frank/H. Mühl
Bachmann M, Scheiermann P, Härdle L, Pfeilschifter J, Mühl H.
IL-36?/IL-1F9, an Innate T-bet Target in Myeloid Cells. J Biol Chem. 2012 Dec 7;287(50):41684-96. doi: 10.1074/jbc.M112.385443.

Kronenberger B, Rudloff I, Bachmann M, Brunner F, Kapper L, Filmann N, Waidmann O, Herrmann E, Pfeilschifter J, Zeuzem S, Piiper A, Mühl H.
Interleukin-22 predicts severity and death in advanced liver cirrhosis: a prospective cohort study. BMC Med. 2012 Sep 11;10:102.

Rudloff I, Bachmann M, Pfeilschifter J, Mühl H.
Mechanisms of rapid induction of interleukin-22 in activated T cells and its modulation by cyclosporin a. J Biol Chem. 2012 Feb 10;287(7):4531-43.

Bingold TM, Ziesché E, Scheller B, Sadik CD, Franck K, Just L, Sartorius S, Wahrmann M, Wissing H, Zwissler B, Pfeilschifter J, Mühl H.
Interleukin-22 detected in patients with abdominal sepsis. Shock. 2010 Oct;34(4):337-40.

Bachmann M, Horn K, Rudloff I, Goren I, Holdener M, Christen U, Darsow N, Hunfeld KP, Koehl U, Kind P, Pfeilschifter J, Kraiczy P, Mühl H.
Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1. PLoS Pathog. 2010 Oct 14;6(10):e1001144.

Ziesché E, Scheiermann P, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Mühl H.
Dexamethasone suppresses IL-22 production associated with bacterial infection in vitro and in vivo, Clin Exp Immunol. 157:370-376, 2009.

Goren I, Müller E, Schiefelbein D, Christen U, Pfeilschifter J, Mühl H, Frank S.
Systemic anti-TNFalpha treatment restores diabetes-impaired skin repair in ob/ob mice by inactivation of macrophages. J Invest Dermatol 127, 2259-2267, 2007.

Schiefelbein D, Goren I, Fisslthaler B, Schmidt H, Geisslinger G, Pfeilschifter J, Frank S.
Biphasic regulation of HMG CoA reductase expression and activity during wound healing and ist functional role in the control of keratinocyte angiogenic and proliferative responses. J Biol Chem 283, 15479-15490, 2008.

Schiefelbein D, Seitz O, Goren I, Dissmann J, Schmidt H, Bachmann M, Sader R, Geisslinger G, Pfeilschifter J, Frank S.
Keratinocyte-derived vascular endothelial growth factor biosynthesis represents a pleiotropic side effect of peroxisome proliferator-activated receptor-gamma agonist troglitazone but not rosiglitazone and involves activation of p38 mitogen-activated protein kinase: implications for diabetes-impaired skin repair. Mol Pharmacol 74, 952-963, 2008.

Goren I, Müller E, Schiefelbein D, Gutwein P, Seitz O, Pfeilschifter J, Frank S.
Akt1 controls insulin-driven VEGF biosynthesis from keratinocytes: implications for normal and diabetes-impaired skin repair in mice. J Invest Dermatol 129, 752-764, 2009.

Ziesche E, Bachmann M, Kleinert H, Pfeilschifter J, Mühl H.
The IL-22/STAT3 pathway potentiates expression of iNOS in human carcinoma cells. J Biol Chem. 282:16006, 2007.

Goren I, Kämpfer H, Müller E, Schiefelbein D, Pfeilschifter J, Frank S.
Oncostatin M expression is functionally connected to neutrophils in the early inflammatory phase of skin repair: implications for normal and diabetes-impaired healing. J Invest Dermatol 126, 628-637, 2006.



P. Gutwein
Gutwein P, Schramme A, Abdel-Bakky MS, Doberstein K, Hauser IA, Ludwig A, Altevogt P, Gauer S, Hillmann A, Weide T, Jespersen C, Eberhardt W, Pfeilschifter J.
ADAM10 is expressed in human podocytes and found in urinary vesicles of patients with glomerular kidney diseases. J Biomed Sci. 2010 Jan 13;17:3.

Lee SB, Doberstein K, Schramme A, Dummer R, Abdel-Bakky MS, Keller S, Altevogt P, Oh ST, Reichrath J, Oxmann D, Pfeilschifter J, Mihic-Probst D, Gutwein P.
ADAM10 is upregulated in melanoma metastasis compared with primary melanoma.
J Invest Dermatol. 2010 Mar;130(3):763-73. Epub 2009 Oct 29



A. Piiper

Interleukin-22 serum levels are a negative prognostic indicator in patients with hepatocellular carcinoma.
Waidmann O1, Kronenberger B, Scheiermann P, Köberle V, Mühl H, Piiper A.
Hepatology. 2014 Mar;59(3):1207. doi: 10.1002/hep.26528. Epub 2013 Dec 13.

Biodegradable human serum albumin nanoparticles as contrast agents for the detection of hepatocellular carcinoma
by magnetic resonance imaging.
Watcharin W1, Schmithals C2, Pleli T2, Köberle V2, Korkusuz H3, Huebner F4, Zeuzem S1, Korf HW5, Vogl TJ4, Rittmeyer C6, Terfort A6, Piiper A1, Gelperina S7, Kreuter J8. Eur J Pharm Biopharm.
2013 Dec 21. pii: S0939-6411(13)00392-5. doi: 10.1016/j.ejpb.2013.12.010. [Epub ahead of print]

Differential Stability of Cell-Free Circulating microRNAs: Implications for Their Utilization as Biomarkers.
Verena Köberle, Thomas Pleli, Christian Schmithals, Eduardo Augusto Alonso, Jörg Haupenthal, Halvard Bönig, Jan Peveling-Oberhag, Ricardo M. Biondi, Stefan Zeuzem, Bernd Kronenberger, Oliver Waidmann, Albrecht Piiper Research Article | published 20 Sep 2013 | PLOS ONE 10.1371/journal.pone.0075184

Transferrin-coated gadolinium nanoparticles as MRI contrast agent.
Korkusuz H, Ulbrich K, Welzel K, Koeberle V, Watcharin W, Bahr U, Chernikov V, Knobloch T, Petersen S, Huebner F, Ackermann H, Gelperina S,
Kromen W, Hammerstingl R, Haupenthal J, Gruenwald F, Fiehler J, Zeuzem S, Kreuter J, Vogl TJ, Piiper A.
Mol Imaging Biol. 2013 Apr;15(2):148-54. doi: 10.1007/s11307-012-0579-6.

Bone morphogenetic protein-9 induces epithelial to mesenchymal transition in hepatocellular carcinoma cells.
Li Q1, Gu X, Weng H, Ghafoory S, Liu Y, Feng T, Dzieran J, Li L, Ilkavets I, Kruithof-de Julio M, Munker S, Marx A, Piiper A, Augusto Alonso E,
Gretz N, Gao C, Wölfl S, Dooley S, Breitkopf-Heinlein K.
Cancer Sci. 2013 Mar;104(3):398-408. doi: 10.1111/cas.12093. Epub 2013 Feb 13.

Interleukin-22 predicts severity and death in advanced liver cirrhosis: a prospective cohort study.
Kronenberger B1, Rudloff I, Bachmann M, Brunner F, Kapper L, Filmann N, Waidmann O, Herrmann E, Pfeilschifter J, Zeuzem S, Piiper A, Mühl H.
BMC Med. 2012 Sep 11;10:102. doi: 10.1186/1741-7015-10-102.

Serum MicroRNA-122 Predicts Survival in Patients with Liver Cirrhosis
Oliver Waidmann, Verena Köberle, Friederike Brunner, Stefan Zeuzem, Albrecht Piiper, Bernd Kronenberger
Research Article | 28 Sep 2012 | doi:10.1371/journal.pone.0045652

Contrast enhancement of the brain by folate-conjugated Gd-DTPA-human serum albumin nanoparticles by magnetic resonance imaging.
Korkusuz, H., Ulbrich, K., Bihrer V., Welzel, K., Chernikov, V., Knobloch, T., Petersen, S., Huebner, F., Ackermann, H., Gelperina, S., Korkusuz, Y.,
Kromen, W., Hammerstingl, R., Haupenthal, J., Fiehler, J., Zeuzem, S., Vogl, T. J., Kreuter, J., and, Piiper, A.
(2012) Mol. Imaging 11, 272-279, 2012

Serum microRNA-122 as predictive marker in patients with liver cirrhosis.
Waidmann, O., Köberle, V., Brunner, F., Zeuzem, S., Piiper, A.*, and Kronenberger, B.*
(2012) PLoS ONE 7, e45652, 2012 *Shared senior authorship

Reduced efficacy of the Plk1 inhibitor BI 2536 on the progression of hepatocellular carcinoma due to low intratumoral drug levels.
Haupenthal J1, Bihrer V, Korkusuz H, Kollmar O, Schmithals C, Kriener S, Engels K, Pleli T, Benz A, Canamero M, Longerich T, Kronenberger B,
Richter S, Waidmann O, Vogl TJ, Zeuzem S, Piiper A.
Neoplasia. 2012 May;14(5):410-9.

Serum MicroRNA-21 as Marker for Necroinflammation in Hepatitis C Patients with and without Hepatocellular Carcinoma.
Verena Bihrer, Oliver Waidmann, Mireen Friedrich-Rust, Nicole Forestier, Simone Susser, Jörg Haupenthal, Martin Welker, Ying Shi,
Jan Peveling-Oberhag, Andreas Polta, Michael von Wagner, Heinfried H. Radeke, Christoph Sarrazin, Jörg Trojan, Stefan Zeuzem,
Bernd Kronenberger, Albrecht Piiper
Research Article | 31 Oct 2011 | PLoS ONE
doi:10.1371/journal.pone.0026971

L. Schaefer

Biglycan: a multivalent proteoglycan providing structure and signals.
Nastase MV, Young MF, Schaefer L.,
J Histochem Cytochem. 2012 Dec;60(12):963-75. doi: 10.1369/0022155412456380. Epub 2012 Jul 20.

International Book Edition: Extracellular Matrix: Pathobiology and Signaling
(Nikos Karamanos Editor), ISBN 978-3-11-025876-9 (2012), DeGruyter, Berlin/Boston. (co-author at one chapter)



T. Tonn, E. Seifried

Engineered factor IX variants bypass FVIII and correct hemophilia A phenotype in mice.
Milanov,P., Ivanciu,L., Abriss,D., Quade-Lyssy,P., Miesbach,W., Alesci,S., Tonn,T., Grez,M., Seifried,E., Schüttrumpf,J.
2012. Blood. 119:602-11

Chemical chaperones improve protein secretion and rescue mutant factor VIII in mice with hemophilia
Roth S.D.*, Schüttrumpf J.*, Milanov P., Abriss D., Ungerer C., Quade-Lyssy P., Simpson J.C., Pepperkok R., Seifried E., Tonn T. A.
PLoS One. 7(9):e44505.  2012

Protein scaffold and expression level determine antiviral activity of membrane-anchored antiviral peptides.
Hermann FG, Martinius H, Egelhofer M, Zahn R, Tonn T, Roth SD, Schult-Dietrich P, Dietrich U, Alexandrov A, Baum C, von Laer D.
Hum. Gene Ther. Epub, 2008 Dec 29.

Non-viral gene transfer results in therapeutic factor IX levels in haemophilia B mice.
Schüttrumpf J, Milanov P, Roth S, Seifried E, Tonn T.
Hamostaseologie. Oct;28 Suppl 1:S92-5, 2008.

Oxygen-induced changes in hemoglobin expression in Drosophila.
Gleixner E, Abriss D, Adryan B, Kraemer M, Gerlach F, Schuh R, Burmester T, Hankeln T.
FEBS Journal, 275(20):5108-16, 2008.

Characterization of neutralizing epitopes within the major capsid protein of human papillomavirus type 33.
Roth SD, Sapp M, Streeck RE, Selinka HC.
Virology Journal 3:83, 2006.



Fachbereich 14: Biochemie, Chemie und Pharmazie


J. Engels
Rehm S, Han S, Hassani I, Sokocevic A, Jonker HR, Engels JW, Schwalbe H.
The high resolution NMR structure of parvulustat (Z-2685) from Streptomyces parvulus FH-1641: comparison with tendamistat from Streptomyces tendae 4158; ChemBioChem,10 (1):119-27, 2009.



Wittel G, Bats JW, Engels JW.
”3´,5´-Bis(tert-butyldimethylsilyl)-5-iodo-2´-deoxy-cytidine”, Acta Cryst., E63m o2657-o2659, 2007.

Wittel G, Gu P, Engels J W.
“Synthesis and Activity of C-Analogues for Immune Stimulating CpG-Oligodeoxynucleotides”, Collection Symposium Series, 7: 493-494, 2005.


D. Steinhilber
Lindner SC, Köhl U, Maier TJ, Steinhilber D, Sorg B.
TLR2 ligands augment cPLA2α activity and lead to enhanced leukotriene release in human monocytes. J. Leuk. Biol., in Revision.




Georg-Speyer-Haus


M. Grez
Nils Pfaff, Nico Lachmann Mania Ackermann, Saskia Kohlscheen, Christian Brendel, Tobias Maetzig, Michael Antoniou, Manuel Grez, Axel Schambach, Thomas Moritz
A Ubiquitous Chromatin Opening Element (UCOE) Prevents Transgene Silencing in Pluripotent Stem Cells and Their Differentiated Progeny. Stem Cells, 2013, accepted for publication

Sofie Vets, Jan De Rijck, Christian Brendel, Manuel Grez, Frederic Bushman, Zeger Debyser and Rik Gijsbers
Transient expression of LEDGF/p75 chimera retargets lentivector integration and functionally rescues a model for X-CGD. Mol Ther., 2013 accepted for publication

Christian Brendel, Walther Hänseler, Vital Wohlgensinger, Matteo Bianchi, Serap Tokmak, Linping Chen-Wichmann, Elena Kouzmenco, Nikola Cesarovic, Flora.Nicholls, Janine Reichenbach, Manuel Grez, Reinhard Seger and Ulrich Siler
Human miR223 Promoter as Novel Myelospecific Promoter for CGD Gene Therapy. Hum. Gene Ther. Methods, 2013, accepted for publication

Kerstin B. Kaufmann, Christian Brendel, Julia D. Suerth, Uta Mueller-Kuller; Linping Chen-Wichmann, Joachim Schwaeble, Shweta Pahujani, Hana Kunkel, Axel Schambach, Christopher Baum and Manuel Grez
Alpharetroviral Vector-mediated Gene Therapy for X-linked Chronic Granulomatous Disease: Functional Correction and Lack of Aberrant SplicingMol Ther., 2012 Dec 4. doi: 10.1038/mt.2012.249. [Epub ahead of print]

Brendel C, Müller-Kuller U, Schultze-Strasser S, Stein S, Chen-Wichmann L, Krattenmacher A, Kunkel H, Dillmann A, Antoniou MN, Grez M.
Physiological regulation of transgene expression by a lentiviral vector containing the A2UCOE linked to a myeloid promoter. Gene Ther. 2012 Oct;19(10):1018-29

Julia D. Suerth, Tobias Maetzig, Martijn H. Brugman, Niels Heinz, Jens-Uwe Appelt, Kerstin B. Kaufmann, Manfred Schmidt, Manuel Grez, Ute Modlich, Christopher Baum and Axel Schambach
Suerth JD, Maetzig T, Brugman MH, Heinz N, Appelt JU, Kaufmann KB, Schmidt M, Grez M, Modlich U, Baum C, Schambach A. Alpharetroviral self-inactivating vectors: long-term transgene expression in murine hematopoietic cells and low genotoxicity.
Mol Ther. 2012 May;20(5):1022-32. doi: 10.1038/mt.2011.309. Epub 2012 Feb 14.

Sean Knight, Fang Zhang, Uta Mueller-Kuller, Marieke Bokhoven, Abhinav Gupta, Thomas Broughton, Sha Sha, Michael Antoniou, Christian Brendel, Manuel Grez,Adrian J Thrasher, Mary Collins, Yasuhiro Takeuchi
Optimisation of ubiquitous chromatin opening element (UCOE) regulated lentiviral vectors through elimination of aberrant splicing. J Virol., 2012 Sep;86(17):9088-95

Giorgia Santilli*, Elena Almarza*, Christian Brendel*, Uimook Choi, Chiara Beilin, Michael P. Blundell, Sneha Haria, Kathryn L Parsley, Christine Kinnon, Harry L. Malech, Juan A Bueren, Manuel Grez and Adrian J Thrasher
Correction of X-CGD by a lentiviral vector containing a novel chimeric promoter driving high gp91phox expression levels in myeloid cells. Mol Ther. 2011 Jan; 19(1):122-32
*equal contribution

Vafaizadeh V, Klemmt P, Brendel C, Weber K, Doebele C, Britt K, Grez M, Fehse B, Desriviéres S, Groner B.
Mammary epithelial reconstitution with gene-modified stem cells assigns roles to Stat5 in luminal alveolar cell fate decisions, differentiation, involution, and mammary tumor formation. Stem Cells, 2010, 28:928-938.

Janine Kimpel, Stephen E Braun, Gang Qiu, Fay Eng Wong, Michelle Connole, Jörn E. Schmitz, Christian Brendel, Laurent M. Humeau, Boro Dropulic, John J. Rossi, Annemarie Berger, Paul Johnson, Dorothee von Laer
Survival of the Fittest: Positive Selection of CD4+ T cells Expressing a Membrane-bound Fusion Inhibitor following HIV-1 Infection. PLoS ONE, 2010 August; 5(8): e12357

Wichmann C, Becker Y, Grez M.
Intracellular expression of peptides. Buchbeitrag in: Groner (Ed.): Peptides as Drugs - Discovery and Development

Stein S, Ott MG, Schultze-Strasser S, Jauch A, Burwinkel B, Kinner A, Schmidt M, Krämer A, Martin H, Glimm H, Koehl U, Preiss C, Göhring G, Schwarzwaelder K, Hofmann WK, Bleuel K, Tchatchou S, Yang R, Reinecke P, Kühlcke K, Schlegelberger B, Thrasher AJ, Hoelzer D, Seger R, von Kalle C, Grez M.
Genomic Instability and Myelodysplasia with Monosomy 7 caused by EVI1 Activation after Gene Therapy for Chronic Granulomatous Disease, Nat Med. 2010, 16:198-204.

Vogler I, Newrzela S, Hartmann S., Schneider N, von Laer D, Koehl U, Grez M.
Adoptive T cell Immunotherapy with an Improved Bicistronic CD20 Vector for Efficient Purification and in vivo Depletion of Gene-modified T Cells. Mol Ther. 2010, in press.

Wichmann C., Becker Y., Chen-Wichmann L., Vogel V., Vojtkova A., Herglotz J., Moore S., Koch J., Lausen J., Mäntele W., Gohlke H., Grez M.
Dimer-tetramer transition controls AML1/ETO leukemogenic activity.
Blood, 2010, in press.

Isabel Vogler, Sebastian Newrzela, Sylvia Hartmann, Nadine Schneider, Dorothee von Laer, Ulrike Koehl and Manuel Grez
An Improved Bicistronic CD20/tCD34 Vector for Efficient Purification and In Vivo Depletion of Gene-Modified T Cells for Adoptive Immunotherapy.
Molecular Therapy (2010); doi:10.1038/mt.2010.83



B. Groner
Borghouts C, Weiss A.
Production and purification of monomeric recombinant peptides for efficient intracellular uptake
and target inhibition. In: Peptides as Drugs - Discovery and Development (Ed. Groner, B.), Wiley-VCH, 2009.



D. von Laer/U. Dietrich
Identification of a new epitope for HIV neutralizing antibodies in the gp41membrane proximal external region by
an Env-tailored phage display library.
Zhou M, Meyer T, Koch S, Koch J, Brill B, von Briesen H, Benito JM, Soriano V, Haberl A, Bickel M, Dübel S, Hust M, Dietrich U.
Eur J Immunol 2012, doi. 10.1002/eji.201242974.

Phage epitope conjugates induce neutralizing antibodies against HIV-1 in prime-boost approaches.
Zhou M*, Kostoula I*, Brill B, Panou E, Sakarellos-Daitsiotis M, Dietrich U.
Vaccine 2012, 30: 1911-16. (*equal contribution)

Inhibition of HIV-1 entry: multiple keys to close the door.
Hertje M, Zhou M, Dietrich U.
ChemMedChem 2010, 5 (11): 1825-35.

A Rationally Engineered Anti-HIV Peptide Fusion Inhibitor with Greatly Reduced Immunogenicity.
Brauer F, Schmidt K, Zahn RC, Richter C, Radeke HH, Schmitz JE, von Laer D, Egerer L.
Antimicrob Agents Chemother. 2013 Feb;57(2):679-88. doi: 10.1128/AAC.01152-12. Epub 2012 Nov 12

Comparison of three humanized mouse models for adoptive T cell transfer.
Volk A, Hartmann S, Muik A, Geiss Y, Königs C, Dietrich U, von Laer D, Kimpel J.
J Gene Med. 2012 Aug;14(8):540-8. doi: 10.1002/jgm.2652.

T-cell receptor diversity prevents T-cell lymphoma development.
Newrzela S,Al-Ghaili N, Heinrich T, Petkova M, Hartmann S, Rengstl B, Kumar A, Jäck HM, Gerdes S, Roeder I, Hansmann ML, von Laer D.
Leukemia. 2012 Dec;26(12):2499-507. doi: 10.1038/leu.2012.142. Epub 2012 May 30.

Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model.
Vets S, Kimpel J, Volk A, De Rijck J, Schrijvers R, Verbinnen B, Maes W, Von Laer D, Debyser Z, Gijsbers R.
Mol Ther. 2012 May;20(5):908-17. doi: 10.1038/mt.2012.6. Epub 2012 Feb 14.

Semireplication-competent vesicular stomatitis virus as a novel platform for oncolytic virotherapy.
Muik A, Dold C, Geiß Y, Volk A, Werbizki M, Dietrich U, von Laer D.
J Mol Med (Berl). 2012 Aug;90(8):959-70. doi: 10.1007/s00109-012-0863-6.

Retroviral insertional mutagenesis can contribute to immortalization of mature T lymphocytes.
Newrzela S, Cornils K, Heinrich T, Schläger J, Yi JH, Lysenko O, Kimpel J, Fehse B, von Laer D.
Mol Med. 2011;17(11-12):1223-32. doi: 10.2119/molmed.2010.00193. Epub 2011 Jul 27.

Cancer suicide gene therapy with TK.007: superior killing efficiency and bystander effect.
Preuss E, Muik A, Weber K, Otte J, von Laer D, Fehse B.
J Mol Med (Berl). 2011 Nov;89(11):1113-24. doi: 10.1007/s00109-011-0777-8. Epub 2011 Jun 23.

Pseudotyping vesicular stomatitis virus with lymphocytic choriomeningitis virus glycoproteins enhances infectivity for glioma cells and minimizes neurotropism.
Muik A, Kneiske I, Werbizki M, Wilflingseder D, Giroglou T, Ebert O, Kraft A, Dietrich U, Zimmer G, Momma S, von Laer D.
J Virol. 2011 Jun;85(11):5679-84. doi: 10.1128/JVI.02511-10. Epub 2011 Mar 30.

Secreted antiviral entry inhibitory (SAVE) peptides for gene therapy of HIV infection.
Egerer L, Volk A, Kahle J, Kimpel J, Brauer F, Hermann FG, von Laer D.
Mol Ther. 2011 Jul;19(7):1236-44. doi: 10.1038/mt.2011.30. Epub 2011 Mar 1.

Survival of the fittest: positive selection of CD4+ T cells expressing a membrane-bound fusion inhibitor following HIV-1 infection.
Kimpel J, Braun SE, Qiu G, Wong FE, Conolle M, Schmitz JE, Brendel C, Humeau LM, Dropulic B, Rossi JJ, Berger A, von Laer D, Johnson RP.
PLoS One. 2010 Aug 23;5(8):e12357. doi: 10.1371/journal.pone.0012357.

Mutations in gp120 contribute to the resistance of human immunodeficiency virus type 1 to membrane-anchored C-peptide maC46.
Hermann FG, Egerer L, Brauer F, Gerum C, Schwalbe H, Dietrich U, von Laer D.
J Virol. 2009 May;83(10):4844-53. doi: 10.1128/JVI.00666-08. Epub 2009 Mar 11.

Transfer of autologous gene-modified T lymphocytes in HIV-infected patients with severe immunodeficiency.
Van Lunzen, J, Glaunsinger T, Stahmer I, von Baehr V, Baum C, Brendel C, Schilz A, Kuehlcke K, Naundorf S, Martinius H, Hermann F, Giroglou T, Newrzela S, Choi I, Brauer F, Brandenburg G, Alexandrov A, von Laer D.
Mol. Ther. 15:1024-1033, 2007.





W. Wels
Jabulowsky RA, Oberoi P, Bähr-Mahmud H, Dälken B, Wels WS.
Surface charge-modification prevents sequestration and enhances tumor-cell specificity of a recombinant Granzyme B - TGFα fusion protein Bioconjugate Chem. 23: 1567-1576, 2012.

Burkhardt UE, Jakobi V, Sloots A, Wei WZ, Cavallo F, Kloke BP, Wels WS.
IL-15 augments antitumoral activity of an ErbB2/HER2 cancer vaccine targeted to professional antigen presenting cells. Cancer Immunol. Immunother., 61: 1473-1484, 2012.

Sahm C, Schönfeld K, Wels WS.
Expression of IL-15 in NK cells results in rapid enrichment and selective cytotoxicity of gene-modified effectors that carry a tumor-specific antigen receptor. Cancer Immunol. Immunother., 61: 1451-1461, 2012.

Meier R, Golovko D, Tavri S, Henning TD, Knopp C, Piontek G, Rudelius M, Heinrich P, Wels WS, Daldrup-Link H.
Depicting adoptive immunotherapy for prostate cancer in an animal model with magnetic resonance imaging. Magn. Reson. Med. 65: 756-763, 2011.

Dälken B, Jabulowsky RA, Oberoi P, Benhar I, Wels WS.
Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein. PLoS ONE 5: e14404, 2010.

Jha P, Reinhart V, Knopp C, Li S, Wels WS, Daldrup-Link H.
NK-cell tracking using non-invasive imaging modalities. in: Natural killer cells, Eds.: Lotze M, Thomson A. Elsevier, im Druck.

Mahmud H, Dälken B, Wels WS.
Induction of programmed cell death in ErbB2/HER2-expressing cancer cells by targeted delivery of apoptosis inducing factor AIF. Mol. Cancer Ther., Mol Cancer Ther. 2009 Jun;8(6):1526-35. Epub 2009 Jun 9.

Tavri S, Jha P, Meier R, Henning TD, Müller T, Hostetter D, Knopp C, Johannson M, Reinhart V, Boddington S, Sista A, Wels WS, Daldrup-Link HE.
Optical imaging of cellular immunotherapy against prostate cancer. Mol. Imaging 2009, im Druck.

Sloots A, Mastini C, Rohrbach F, Weth R, Curcio C, Burkhardt U, Jäger E, Forni G, Cavallo F, Wels WS.
DNA vaccines targeting tumor antigens to B7 molecules on APCs induce protective anti-tumor immunity and delay onset of HER2/Neu-driven mammary carcinoma. Clin. Cancer Res. 14: 6933-6943, 2008.

Giesübel U, Dälken B, Mahmud H, Wels WS.
Cell binding, internalization and cytotoxic activity of human granzyme B expressed in the yeast Pichia pastoris. Biochem. J. 394: 563-573, 2006.



M. Zörnig
Gutschner, T., Hämmerle, M., Eißmann, M., Hsu, J., Kim, Y., Hung, G., Revenko, A.S., Arun, G., Stentrup, M., Groß, M., Zörnig, M., Macleod, A.R., Spector, D.L., and Diederichs, S.
The non-coding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells.
Cancer Res. 2012, Dec 14 [Epub ahead of print].

Eißmann, M., Gutschner, T., Hämmerle, M., Günther, S., Caudron-Herger, M., Groß, M., Schirmacher, P., Rippe, K., Braun, T., Zörnig, M1. and Diederichs, S1 (2012).
Loss of the abundant nuclear non-coding RNA MALAT1 is compatible with life and development.
1 both senior authors contributed equally to the work
RNA Biol. 9, 1076-87.

Rabenhorst, U., Beinoraviciute-Kellner, R., Brezniceanu, M.-L., Joos, S., Devens, F., Lichter, P., Rieker, R. J., Trojan, J., Chung, H.-J., Levens, D. L., and Zörnig, M. (2009).
Overexpression of the Far Upstream Element Binding Protein FBP1 in hepatocellular carcinoma is required for tumor growth.
Hepatology 50, 1121-9.

Paunel-Görgülü, A., Zörnig, M., Lögters, T., Altrichter, J, Rabenhorst, U., Cinatl, J., Windolf, J., and Scholz, M. (2009).
Mcl-1-mediated impairment of the intrinsic apoptosis pathway in circulating neutrophils from critically ill patients can be overcome by Fas stimulation.
J Immunol. 183, 6198-206.





Paul-Ehrlich-Institut



K. Cichutek/C. Buchholz/M. Schweizer
Funke S, Schneider IC, Glaser S, Mühlebach MD, Moritz T, Cattaneo R, Cichutek K, Buchholz CJ.
Pseudotyping lentiviral vectors with the wildtype measles virus glycoproteins improves titer and selectivity. Gene Therapy, Epub, 12 February 2009.

Funke S, Maisner A, Mühlebach MD, Koehl U, Grez M, Cattaneo R, Cichutek K, Buchholz CJ.
Targeted cell entry of lentiviral vectors. Molecular Therapy, Vol. 16 (8): 1427-1436, 2008.

Buchholz CJ, Duerner LJ, Funke S, Schneider IC.
Retroviral Display and High Throughput Screening. Combinatorial Chemistry & High Throughput Screening, Vol. 11 (2): 99-110, 2008.

Europäische Patentanmeldung am 27.09.2006 (06020257.9; Veröffentlichungsnr. 1975239) und PCT-Anmeldung am 26.09.2007 (PCT/EP2007/008384; Veröffentlichungsnr. WO 2008/037458 A3) mit dem Titel „Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high-throughput screening”
Erfinder: Buchholz CJ, Funke S, Cichutek K, Cattaneo R.

Mühlebach MD, Schüle S, Kaiser JK, Kloke B-P, Cichutek K, Schweizer M
Impact of viral accessory proteins of SIVsmmPBj on early steps of infection of quiescent cells. Wolfrum N, Virology 364: 330-341, 2007.


Barbara Schnierle
Kraus B, Mönk B, Sliva K, Schnierle BS
Expression of Human Endogenous Retrovirus-K Coincides with that of Micro-RNA-663 and -638 in Germ Cell Tumor Cells.
Anticancer Research, 32:4797-804 (2012)

Sliva K, Resch T, Kraus B, Goffinet C, Keppler OT, Schnierle BS.
The cellular antiviral restriction factor tetherin does not inhibit poxviral replication.
J Virol., 86:1893-6. (2012)

Kraus B, Boller K, Reuter A, Schnierle BS.
Characterization of the human
endogenous retrovirus K Gag protein: identification of protease cleavage sites.
Retrovirology; 8:21. (2011)

C. Kamp, T. Wolf, I. G. Bravo, B. Kraus, B. Krause, B. Neumann, G. Winskowsky, A. Thielen, A. Werner and Schnierle B. S.
Decreased HIV diversity after allogeneic stem cell transplantation of an HIV-1 infected patient.
Virol J, 7:55 (2010)          

Kremer M, Klemke C-D and Schnierle BS
Cutaneous T-cell lymphoma cells are sensitive to rapamycin.
Experimental Dermatology, 19:800-805 (2010)                                                                               

Frei G, Kremer M, Hanschmann K, Krause S, Albeck M, Sredni B, Schnierle BS.
Antitumour effects in mycosis fungoides of the immunomodulatory, tellurium-based compound, AS101. British Journal of Dermatology 158: 578-586, 2008.

Kremer M, Suezer Y, Martinez-Fernandez Y, Munk C, Sutter G, Schnierle BS.
Vaccinia virus replication is not affected by APOBEC3 family members. Virol. J. 3: 86, 2006.

Kremer M, Bittner A, Schnierle BS.
Human APOBEC3G incorporation into murine leukemia virus particles. Virology 337: 175-182, 2005.

Kremer M, Schnierle BS.
HIV-1 Vif: HIV’s weapon against the cellular defense factor APOBEC3G. Curr. HIV. Res. 3: 339-344, 2005.